Dr. Christian Wölk
web links
ORCID ID: orcid.org/0000- 0002- 8067- 7307
Research Group - Nucleic Acid-based Therapies
Group Leader:
- Dr. Christian Wölk
PhD Students:
- Dipl. Pharm. Julia Giselbrecht
- Dipl. Pharm. Catharina Husteden
Diploma Student:
- Thomas Guttenberg (collaboration research group Prof. Dailey)
Development of cationic lipid composites as nucleic acid delivery systems
comming soon
Funding
Deutsche Forschungsgemeinschaft (DFG) - Grand Number 396823779
Development of lipoplex-loaded model surface coatings for the in-situ transfection in the field of bone regeneration
Prizes/Honors
Finalist in der Rubrik "Innovativste Vorhaben der Grundlagenforschung" des Hugo Junkers Preis 2017 [more]
Science Prize of the Bayer Bitterfeld GmbH 2015 [more]
Luther-Urkunde of the Martin Luther University Halle-Wittenberg - defense of the PhD thesis with Summa cum laude
excellence scholarshop (Gesetz zur Förderung des wissenschaftlichen und künstlerischen Nachwuchses
[Graduiertenförderungsgesetz - Grad FG] in der Fassung der Bekanntmachung vom 30. Juli 2001)
Publications
28. C. Husteden, F. Doberenz, N. Goergen, S. R. Pinnapireddy, C. Janich, A. Langner, F. Syrowatka, A. Repanas, F. Erdmann, J. Jedelská, U. Bakowsky, T. Groth, C. Wölk. Contact-Triggered Lipofection from Multilayer Films Designed as Surfaces for In Situ Transfection Strategies in Tissue Engineering. ACS Appl. Mater. Interfaces 2020, https://doi.org/10.1021/acsami.9b18968
27. J. J. Schüer, C. Wölk, U. Bakowsky, S. R. Pinnapireddy. Comparision of Tanaka lipid mixture with natural surfactant Alveofact to study nanoparticle interactions on Langmuir film balance. Colloids and Surfaces B. 2020, 188, 110750 [more]
25. C. Wölk, G. Hause, O. Gutowski, R. D. Harvey, G. Brezesinski. Enhanced chain packing achieved via putative headgroup ion-triplet formation in binary anionic lipid/cationic surfactant mixed monolayers. Chemistry and Physics of Lipids 2019, 255, 104827 [more]
24. J. Giselbrecht, S. Wiedemann, S. R. Pinnapireddy, N. Goergen, H. Loppnow, D. Sedding, F. Erdmann, U. Bakowsky, G. Hause, M. Lúcio, A. Langner, C. Wölk. Nucleic acid carrier composed of a branched fatty acid lysine conjugate—Interaction studies with blood components. Coll. Surf. B. 2019, 184, 110547 [more]
23. C. Stefaniu, C. Wölk, G. Brezesinski, E. Schneck. Relationship between structure and molecular interactions in monolayers of specially designed aminolipids. Nanoscale Advances 2019, 1 (9) , 3529-3536. [more]
22. D. Pawlowska, C. Janich, A. Langner, B. Dobner, C. Wölk, G. Brezesinski. The Impact of Alkyl‐Chain Purity on Lipid‐Based Nucleic Acid Delivery Systems – Is the Utilization of Lipid Components with Technical Grade Justified?ChemPhysChem 2019, 20, 2110. [more]
21. S. Tassler, B. Dobner, L. Lampp, R. Ziółkowski, E. Malinowska, C. Wölk, G. Brezesinski: DNA Delivery Systems Based on Peptide-Mimicking Cationic Lipids—The Effect of the Co-Lipid on the Structure and DNA Binding Capacity. Langmuir 2019, 35, 13, 4613-4625 [more]
20. M. Dittrich, C. Brauer, S.S. Funari, B. Dobner, G. Brezesinski, and C. Wölk: Interactions of Cationic Lipids with DNA – a Structural Approach. Langmuir 2018, 34, 14858-14868. [more]
15. C. Janich, A. Hädicke, U. Bakowsky, G. Brezesinski, and C. Wölk: Interaction of DNA with Cationic Lipid Mixtures—Investigation at Langmuir Lipid Monolayers. Langmuir, 2017, 10172-10183, [more]
14. C. Wölk, C. Janich, U. Bakowsky, A. Langner, and G. Brezesinski: Malonic acid based cationic lipids – The way to highly efficient DNA-carriers. Adv. Coll. Interface Science 2017, 248, 20-34, [more]
13. S. Tassler, C. Wölk, C. Janich, B. Dobner, and G. Brezesinski: Lysine-based amino-functionalized lipids for gene transfection: the protonation state in monolayers at the air–liquid interface. PCCP 2017, 19, 20271-20280, [more]
12. C. Janich, S.R. Pinnapireddy, F. Erdmann, T. Groth, A. Langner, U. Bakowsky, and C. Wölk: Fast therapeutic DNA internalization – a high potential transfection system based on a peptide mimicking cationic lipid. Eur. J. Pharm. Biopharm. 2017, 118, 38-47, [more]
11. C. Janich, S. Taßler, A. Meister, G. Hause, J. Schäfer, U. Bakowsky, G. Brezesinski and C. Wölk: Structures of malonic acid diamide/phospholipid composites and their lipoplexes.
Soft Matter 2016, 12, 5854-5866, [more]
10. C. Janich, C. Wölk, F. Erdmann, T. Groth, G. Brezesinski, B. Dobner, and A. Langner: Composites of malonic acid diamides and phospholipids — Impact of lipoplex stability on transfection efficiency.
Journal of Controlled Release 2015, 220, 295–307. [more]
9. C. Wölk, C. Janich, A. Meister, S. Drescher, A. Langner, G. Brezesinski, and U. Bakowsky: Investigation of Binary Lipid Mixtures of a Three-Chain Cationic Lipid with Phospholipids Suitable for Gene Delivery.
Bioconjugate Chem. 2015, 26 (12), 2461–2473. [more]
8. X. Zhang, L. Dai, A. Wang, C. Wölk, B. Dobner, G. Brezesinski, Y. Tang, X. Wang, and J. Li: The Directional Observation of Highly Dynamic Membrane Tubules Formation Induced by Engulfed Liposomes. Scientific Reports 5, Art. Num. 16559, doi: 10.1038/srep16559 [more]
7. N. Erdmann, C. Wölk, I. Schulze, C. Janich, M. Folz, S. Drescher, M. Dittrich, A. Meister, J. Vogel, T. Groth, B. Dobner, and A. Langner: Tris(2-aminoethyl)-amine-based α-branched fatty acid amides – Synthesis of lipids and comparative study of transfection efficiency of their lipid formulations.
Eur. J. Pharm. Biopharm. 2015, 96, 349-362. [more]
6. C. Wölk, C. Janich, D. Pawlowska, S. Drescher, A. Meister, G. Hause, B. Dobner, A. Langner, and G. Brezesinski: Lamellar versus Micellar Structures - Aggregation Behaviour of a Three-Chain Cationic Lipid Designed for Nonviral Polynucleotide Transfer.
ChemPhysChem 2015, published online. [more]
ChemPhysChem 2015, 16 (10), 2029. (Cover Profile) [more]
5. C. Janich, C. Wölk, S. Taßler, S. Drescher, A. Meister, G. Brezesinski, B. Dobner and A. Langner: Composites of malonic acid diamides and phospholipids - structural parmeters for optimal transfection efficiency in A549 cells.
Eur. J. Lipid Sci. Technol. 2014, 116, 1205-1216. [more]
4. C. Wölk, D. Pawlowska, S. Drescher, A. Auerswald, A. Meister, G. Hause, A. Blume, A. Langner, G. Brezesinski, and B. Dobner: New micellar transfection agents
Langmuir 2014, 30, 4905–4915. [more]
3. C. Wölk, S. Drescher, A. Meister, A. Blume, A. Langner, B. Dobner:
General Synthesis and Physico-Chemical Characterisation of a Series of Peptide-Mimic Lysine-Based Amino-Functionalised Lipids.
Chem. Eur. J. 2013, 19, 12824-12838. [more]
2. M. Dittrich, M. Heinze, C. Wölk, S. S. Funari, B. Dobner, H. Möhwald, and G. Brezesinski: Structure-Function Relationships of New Lipids Designed for DNA Transfection.
ChemPhysChem 2011, 12, 2328-2337. [more]
1. C. Wölk, M. Heinze, P. Kreideweiß, M. Dittrich, G. Brezesinski, A. Langner, and B. Dobner: Synthesis and DNA transfection properties of new head group modified malonic acid diamides.
Int. J. Pharm. 2011, 409, 46-56. [more]
talks
Development of lipoplexloaded model surface coatings for the in situ transfection in the field of bone regeneration. Annual Meeting of the German Pharmaceutical Society - DPhG 2019
Invited talk
BioNanoMed 2019, Graz, Austria
Invited talk
22nd Annual Meeting CRS - German Local Chapter, Leipzig, Germany
Giant plasma membrane vesicles: a model to study passive interactions of nanomedicines with the cell surface. 12th international Conference and Workshop on Biological Barriers 2018
A novel three-chain lysine-based cationic lipid - fast and efficient DNA transfer without co-lipids. Annual Meeting of the German Pharmaceutical Society - DPhG 2017
Peptide Mimicking Lipids in Gene Therapy - Investigation of Stability in Serum.
21st Annual Meeting CRS - German Local Chapter, Marburg, Germany
Lamellar vs. micellar - a tripple-chained cationic lipid as polynucleotide carrier.
Joint Meeting of the Membrane Sections of the French and German Biophysical Societies, Aril 11 to 14 1016, Bad Herrenalb, Germany
Effective Lipid-mediated DNA Transfer with a Three-Chain Amino-Functionalized Lipid Designed for Lipofection
11th international Conference and Workshop on Biological Barriers 2016
Posters
Y.A. Brito Barrera, C. Wölk, T. Groth
Use of cationic liposomes embedded onto polyelectrolyte multilayer system for controlled release
CRS local chapter 2018, Halle
J. Giselbrecht, S.R. Pinnapireddy, U. Bakowsky, A. Langner, C. Wölk
Analysis of the physiological barriers after systemic application of the lipoplexes of T14diLys
CRS local chapter 2018, Halle
M. Alawak, K. Engelhardt, G. Mahmoud, I. Levacheva, J. Büßler, C. Wölk, J.W. Bartsch, U. Bakowsky
Magnetic thermosensitive immunoliposomes loaded with doxorubicin
CRS local chapter 2018, Halle
S. Müller, A. Springwald, E. Bothe, N. Weber, A. Ewe, A. Aigner, C. Wölk, M.C. Hacker, M. Schulz-Siegmund
Controlled release of siRNA from oligomer-crosslinked gelatin microparticles
CRS local chapter 2018, Halle
C. Wölk, C. Janich
Interaction of DNA with Cationic Lipid Mixtures - A fluorescence microscopic study at the air/water interface
Symposium "Phospholipids in Pharmaceutical Research" 2017
J. Giselbrecht, S.R. Pinnapireddy, U. Bakowsky, A. Langner, C. Wölk
Comparativ analyses of lipoplexes regarding their complexation behaviour and stability under physiological conditions
Symposium "Phospholipids in Pharmaceutical Research" 2017
S. Müller, A. Ewe, J. Giselbrecht, C. Wölk, A. Aigner, M.C. Hacker, M. Schulz-Siegmund
Liposome and polymer based siRNA transfection in hASC and SaOS-2 cells
Biotechnology Symposium - University Leipzig 20017
C. Wölk, A. Langner, U. Bakowsky, C. Janich
A New Class of High Potential Nucleic Acid Delivery Systems -
To Fuse or Not to Fuse: That is the Question
NaNo DDS, September 2016, Baltimor, USA
C. Janich, U. Bakowsky, A. Langner, C. Wölk
Fast Therapeutic DNA Internalization –
a New Class of High Potential Nucleic Acid Delivery Systems
NaNo DDS, September 2016, Baltimor, USA
C. Janich, C. Wölk, A. Hädicke, A. Langner
Monolayer Studies of Phospholipid-Based Gene Delivery Systems
Joint Meeting of the Membrane Sections of the French and German Biophysical Societies, Aril 11 to 14 1016, Bad Herrenalb, Germany
C. Janich, C. Wölk, A. Langner
Phospholipid-Based Gene Delivery Systems – DNA complexation and DNA release behavior of lipoplexes
11th nternational Conference and Workshop on Biological Barriers 2016
C. Janich, A. Langner, U. Bakowsky, S. Taßler, G. Brezesinski, B. Dobner, and C. Wölk
Structural Investigations of Cationic Lipid Compositions and their Complexes with DNA
21th Ostwald-Colloquium- MPI-KG Potsdam 2015
C. Janich, C. Wölk, F. Erdmann, T. Groth, G. Brezesinski, B. Dobner, and A. Langner
Phospholipid-Based Gene Delivery Systems – Impact of Lipoplex Stability on Transfection Efficiency
4. Symposium on Phospholipids in Pharmaceutical Research - Heidelberg 2015
C. Wölk, C. Janich, S. Taßler, A. Meister, G. Hause, U. Bakowsky, A. Langner, G. Brezesinski, and B. Dobner
Phospholipid-Based Gene Delivery Systems – Aggregate Structures
4. Symposium on Phospholipids in Pharmaceutical Research - Heidelberg 2015
S. Taßler, C. Janich, C. Wölk, B. Dobner, and G. Brezesinski
Physical-chemical investigation of a new cationic lipid for gene transfection
International Workshop "Molecular Membrane Biophysics" - Hünfeld 2014
D. Pawlowska, C. Wölk, B. Dobner, and G. Brezesinski
The impact of chain purity of cationic lipids on the transfection efficiency
International Workshop "Molecular Membrane Biophysics" - Hünfeld 2014
C. Janich, C. Wölk, S. Taßler, S. Drescher, A. Meister, G. Brezesinski, B. Dobner, and A. Langner
Investigation of Phospholipids in Mixture with Cationic Lipids - Biological and Physicochemical Characterization.
International Workshop "Molecular Membrane Biophysics" - Hünfeld 2014
C. Janich, C. Wölk, B. Dobner, and A. Langner
Evaluation of Transfection Efficiency of Cationic Lipids/Co-lipid-Complexes using an EGFP-Assay
3. Symposium on Phospholipids in Pharmaceutical Research - Heidelberg 2013
C. Wölk, S. Drescher, A. Meister, A. Blume, A. Langner, and B. Dobner
Physicochemical Characterization of the Miscibility of Phospholipids with a Novel Amino-Functionalized Lipid Bearing Three Alkyl Chains
3. Symposium on Phospholipids in Pharmaceutical Research - Heidelberg 2013
C. Wölk, A. Langner, and B. Dobner
Synthesis of Cationic Lipids with Peptide-Like Malonic Acid Diamide Backbone
DPhG-Tagung - Insbruck 2011
P. Kreideweiß, C. Wölk, B. Dobner, and A. Langner
Cationic Lipids for Liposomal Gene Transfer
DPhG-Tagung - Insbruck 2011
C. Wölk, N. Erdmann, S. Drescher, A. Meister, A. Blume, A. Langner, and B. Dobner
Physicochemial Characterization of Cationic Liposomes for the Use as Gene Transfection Systems.
2. Symposium on Phospholipids in Pharmaceutical Research - Heidelberg 2011
C. Wölk, A. Langner, and B. Dobner
Synthesis of Cationic Lipids with a Novel Peptide-like Malonic Diamide Backbone
Frontiers in Medicinal Chemistry - Saarbrücken 2011
C. Wölk, M. Heinze, P. Kreideweiß, B. Dobner, and A. Langner
Synthesis of Novel Cationic Lipids with Malonic Diamide Backbone and Lysine Containing Headgroup
DPhG-Tagung - Braunschweig 2010
Teaching
anorganic chemistry in pharmacy
organic chemistry in pharmacy
instrumental analysis
lecture: Drug quality control -European and international standarts and regulations (master program)