Dipl. Pharm. Catharina Husteden
Development of lipoplex-loaded model surfaces for the in situ transfection in the field of bone regeneration
An interesting field of research and one application of gene therapy is the DNA-induced bone regeneration in the field of regenerative medicine. In the Western World 5 to 10% of bone fractures show insufficient healing which results in a longer leave of absence and higher costs for the health care system. Hence, numerous strategies in the regenerative medicine focus on initiating fast osteogenesis on artificial scaffolds to stimulate the healing of bone fractures. However, these strategies primary use recombinant proteins to induce osteogenesis, which may result in complex biotechnological manufacturing and therefore high therapy costs.
The vision of the project is to produce material films loaded with DNA. Due to gene therapy, the material films induce the local osteogenic differentiation of mesenchymal stem cells which results in rapid bone healing. To realize this vision the proposed project has a number of objectives. First, functionalised material coatings will be developed based on polyelectrolyte multi layers (PEMs). These PEMs are composed of biocompatible polymers and will be loaded with layers of DNA. Due to the highly effective cationic lipid composites the DNA will be complexed. The lipid encapsulation has protective and transfection enhancing effects.
Publications
Y.A. Brito Barrera, C. Husteden, J. Alherz, B. Fuhrmann, C. Wölk, T. Groth
Extracellular matrix-inspired surface coatings functionalized with dexamethasone-loaded liposomes to induce osteo- and chondrogenic differentiation of multipotent stem cells. Materials Science & Engineering C 2021
C. Husteden, T. Groth, C.Wölk
Implantatüberzüge für die in-situ Transfektion in der regenerativen Medizin. BIOspektrum, Volume 20, Issue 4, 2021, [more]
C. Husteden, F. Doberenz, N. Goergen, S. R. Pinnapireddy, C. Janich, A. Langner, F. Syrowatka, A. Repanas, F. Erdmann, J. Jedelská, U. Bakowsky, T. Groth, C. Wölk. Contact-Triggered Lipofection from Multilayer Films Designed as Surfaces for In Situ Transfection Strategies in Tissue Engineering. ACS Appl. Mater. Interfaces 2020, [more]
Conference Contributions
C. Husteden, A. Langner, T. Groth, C. Wölk
In-situ Lipofection with nanostructured Multilayer Systems to mediate BMP-2 gene delivery for bone regeneration
47th European Society for Artificial Organs Congress London (ESAO 2021)
C. Husteden, A. Langner , T. Groth, C. Wölk
Surface-Mediated Gene Delivery from functionalized Polyelectrolyte Multilayer Scaffolds in Tissue Engineering
11th World Biomaterials Congress Glasgow (WBC Virtual 2020)
C. Husteden, F. Doberenz, S.R. Pinapireddy, A. Langner , T. Groth, C. Wölk
Contact-Triggered Transfection from Multilayer-Systems in Tissue Engineering
Symposium Controlled Release Society 2020 (CRS German Chapter München)
C. Husteden, F. Doberenz, S.R. Pinapireddy, A. Repanas, T. Groth, C. Wölk
Gene Delivery from new designed Polyelectrolyte Multilayer Scaffolds in Tissue Engineering
Leipzig Research Festival of Lifescience 2020
C. Husteden, F. Doberenz, S.R. Pinapireddy, A. Repanas, T. Groth, C. Wölk
Functionalized Polyelectrolyte Multilayer Scaffolds as Gene Delivery systems in Tissue Engineering
2nd International workshop on advanced materials for healthcare applications 2019 (IWAMHA Madeira)
C.Husteden, A. Repanas, T. Groth, C. Wölk
DNA-loaded Polyelectrolyte Multilayer Scaffolds for Local Transfection on Bone Structures
Annual Meeting of the German Pharmaceutical Society 2019 (DPhG Heidelberg)
C.Husteden, A. Repanas, T. Groth, C. Wölk
Incorporation of Novel Cationic Lipoplexes in Polyelectrolyte Multilayer Coatings for Controlled Release in the field of Bone Regeneration
International Congress Nanotechnology in Medicine & Biology 2019 (BioNanoMed Graz)
C.Husteden, A. Repanas, T. Groth, C. Wölk
Surface properties and Cell Adhesions Studies of Lipoplex-Loaded Polyelectrolyte Multilayers
Symposium Controlled Release Society 2019 (CRS German Chapter Leipzig)
C. Husteden, T. Groth, C. Wölk
Development of Methods for Effective DNA Loading of Model Surfaces for in-situ Transfection
International Conference and Workshop on Biological Barriers 2018 (BioBarriers Saarbrücken)