M.Sc. Stefan Lange
Synthesis and physico-chemical characterization of perdeuterated ceramides
The skin is the largest organ of the human and has a variety of different functions, e.g. the regulation of the thermal and the water balance.
Another important role is the barrier function of the stratum corneum. The stratum corneum, the outermost layer of the human skin is composed of keratinocytes and an intercellular lipid matrix. The matrix consists of ceramides, cholesterol and free fatty acids. The ceramides play an important role in the molecular structure of the matrix.
With different kinds of physico-chemical methods, e.g. NMR- and IR-spectroscopy, more detailed information about the matrix structure should be received. This knowledge and the comprehension of the lipid structure are necessary for the development of new potential drug delivery systems.
For detailed studies pure ceramides with a defined chain length are essential. The aim of this work is to synthesize different kinds of perdeuterated ceramids and spingolipids.
The measurements are performed by the research group of Prof. Dr. Neubert (Department of Pharmaceutical Technology and Biopharmaceutics, MLU Halle), Prof. Dr. Huster (Institute for Medical Physics and Biophysics, University Leipzig) and Prof. Dr. Brezesinski (Max-Planck-Institute of Colloids and Interfaces, Potsdam-Golm).
Publications
Tessema, Efrem N.; Gebre-Mariam, Tsige; Lange, Stefan; Dobner, Bodo; Neubert, Reinhard H. H.: Potential application of oat-derived ceramides in improving skin barrier function: Part 1. Isolation and structural characterization. Journal of Chromatography B, 2017, 1065-1066, 87-95. [more]
Schmitt, Thomas; Lange, Stefan; Dobner, Bodo; Sonnenberger, Stefan; Hauss, Thomas; Neubert, Reinhard H. H.: Investigation of a CER[NP]- and [AP]-Based Stratum Corneum Modeling Membrane System: Using Specifically Deuterated CER Together with a Neutron Diffraction Approach. Langmuir, 2017, in press. [more]
Stahlberg, Soeren; Eichner, Adina; Sonnenberger, Stefan; Kovacik, Andrej; Lange, Stefan; Schmitt, Thomas; Deme, Bruno; Hauss, Thomas; Dobner, Bodo; Neubert, Reinhard H. H.: Influence of a Novel Dimeric Ceramide Molecule on the Nanostructure and Thermotropic Phase Behavior of a Stratum Corneum Model Mixture. Langmuir 2017, 33(36), 9211-9221. [more]
S. Sonnenberger, A. Eichner, T. Hauß, A. Schroeter, T. Schmitt, T. Hauß, S. Lange, A. Langner, R.H.H. Neubert, B. Dobner: Synthesis of specific deuterated derivatives of the long chained stratum corneum lipids [EOS] and [EOP] and characterization using neutron scattering.
Journal of Labelled Compounds and Radiopharmaceuticals 2017, 60, Pages 316-330. [more]
J.S.L. Oliveira, S. Lange, B. Dobner, G. Brezesinski: Analytical data of synthesized deuterated isopropyl myristate and data about the influence of IPM/IPMdeut on the thermodynamics and morphology of 2D Stratum Corneum models.
Data in Brief 2017, 12, Pages 624–631. [more]
J.S.L. Oliveira, S. Lange, B. Dobner, G. Brezesinski: The effect of non-deuterated and deuterated isopropyl myristate on the thermodynamical and structural behavior of a 2D Stratum Corneum model with Ceramide [AP]. ChemPhys Lipids 2017, 204, 1-9. [more]
A. Eichner, S. Stahlberg, S. Sonnenberger, S. Lange, B. Dobner, A. Ostermann, T.E. Schrader, T. Hauß, A. Schroeter, D. Huster: Influence of the penetration enhancer isopropyl myristate on stratum corneum lipid model membranes revealed by neutron diffraction and 2H NMR experiments.
BBA - Biomembranes 2017, 1859, 745-755. [more]
S. Sonnenberger, S. Lange, A. Langner, R.H.H. Neubert and B. Dobner: Synthesis of ceramides NS and NP with perdeuterated and specifically ω deuterated N-acyl residues.
Journal of Labelled Compounds and Radiopharmaceuticals 2016, 59, 531-542. [more]
S. Stahlberg, S. Lange, B. Dobner, and D. Huster: Probing the Role of Ceramide Headgroup Polarity in Short-Chain Model Skin Barrier Lipid Mixtures by 2H Solid-State NMR Spectroscopy.
Langmuir 2016, 32, 2023-2031. [more]
F. Sahle, S. Lange, B. Dobner, J. Wohlrab, and R. H. H. Neubert: Development and Validation of LC/ESI-MS Method for Detection and Quantification of Exogenous Ceramide NP in Stratum Corneum and Other Layers of the Skin.
J. Pharmaceut. Biomed. 2012, 60, 7-13. [more]
Poster and presentations
S. Sonnenberger, A. Eichner, S. Lange, A. Langner, R.H.H. Neubert, and B. Dobner: Synthesis of a modified deuterated ω-Acyl Ceramide [EOS] Species and the Impact to the Nanostructure of Stratum Corneum Lipid Model Membranes.
DPhG-Tagung - Düsseldorf 2015 (Poster)
S. Lange, S. Sonnenberger, A. Eichner, R.H.H. Neubert, and B. Dobner: Synthesis of specific deuterated ceramide species [NP] and [NS] with different chain lengths.
DPhG-Tagung - Düsseldorf 2015 (Poster)