Small bioactive molecules
As targets or tools, small bioactive molecules play an undisputedly important role in pharmaceutical research. Compounds with nitrogen-containing heterocycles, developed by nature over a long period of biological evolution, possess essential molecular functions. On the one hand, the structures of these connections are characterized by enormous diversity. On the other hand, the conversion of proteins and peptides into small bioactive molecules is of pharmaceutical interest for drug discovery.
Investigation of physicochemical properties of small bioactive molecules and their influences on the body's lipid biosynthesis
In addition to proteins and peptides, molecules with low molecular weight, especially nitrogen-containing compounds, play a special role in the area of drug development. The bioavailability and thus also the effectiveness are decisively influenced by physicochemical parameters. In particular, the distribution behavior under physiological conditions, the acid constant and the solubility are key factors that, among other things, determine the further success of a molecule in pharmaceutical research. Various analytical methods are intended to supplement or even expand computer-aided calculations with the aim of predicting the physicochemical behavior of a molecule in the body as accurately as possible and using this knowledge to take appropriate measures in the development of active ingredients. Chromatography, especially HPLC, in conjunction with UV/Vis analysis is used here. The method development is based on the OECD guidelines. Another important factor is the influence of these small molecules on various physiological and pathophysiological processes. The focus will be on a comprehensive characterization of the lipid mediators formed in arachidonic acid metabolism. Possible effects are observed in stimulated human whole blood. Sample processing is carried out using a method specially developed by the working group, followed by HPLC.